Current technologies for the real-time analysis of biomarkers in vivo, such as needle-type microelectrode and molecular imaging methods based on exogenous contrast agents, are still facing great challenges in either invasive detection or lack of active control of the imaging probes. In this study, by combining the design concepts of needle-type microelectrode and fluorescence imaging method, we have developed a new technique for detecting biomarker in vivo, named as optically controlled virtual microsensor (OCViM). OCViM is established by the organic integration of a specially shaped laser beam and fluorescent nanoprobe, which serve as the virtual handle and sensor tip, respectively. The laser beam can trap and manipulate the nanoprobe in a programmable manner, and meanwhile excite it to generate fluorescence emission for biosensing. On this basis, the fully active control of the nanoprobe was achieved noninvasively in vivo and multipoint detection could be realized at sub-micron resolution by shifting a nanoprobe among multiple positions. By using OCViM, the over-expression and heterogenous distribution of biomarkers in thrombus was studied in living zebrafish, which was further utilized for the evaluation of antithrombotic drugs. OCViM may provide a powerful tool for the mechanism study of thrombus progression and the evaluation of antithrombotic drugs. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.

原文链接：http://www.ncbi.nlm.nih.gov/pubmed/36074977